Evaluation of serum IgE in peach-allergic patients with systemic reaction by using recombinant Pru p 7 (gibberellin-regulated protein)

Main Article Content

Y. Mori
F. Okazaki
C. Inuo
Y. Yamaguchi
S. Masuda
S. Sugiura
T. Fukuie
M. Nagao
I. Tsuge
T. Yosikawa
A. Yagami
K. Matsunaga
T. Fujisawa
K. Ito
H. Narita
Y. Kondo

Keywords

Component-resolved diagnosis (CRD), Fruit allergy, Gibberellin-regulated protein, Lipid transfer protein (LTP)

Abstract

Background: Lipid transfer protein (LTP) is a major fruit allergen. It has, however, recently been revealed that the systemic reaction in peach-allergic patients is related not only to LTP (Pru p 3) but also to gibberellin-regulated protein (Pru p 7). We investigated recombinant Pru p 7 (rPru p 7) for its potential use in worldwide standardization for the diagnosis of peach allergy.


Methods: Natural Pru p 7 (nPru p 7) was purified from peach crude extract using a monoclonal antibody affinity column. Complementary DNA for Pru p 7 was cloned and expressed in Escherichia coli and Pichia pastoris. Serum immunoglobulin (Ig) E in peach-allergic patients was examined by enzyme-linked immunosorbent assay (ELISA) using nPru p 7 and rPru p 7 (E. coli product: erPru p 7 and P. pastoris product: prPru p 7).


Results: Peach-allergic patients (n = 27) were diagnosed and categorized into oral reaction (n = 10) or systemic reaction (n = 17). The nPru p 7 positivity based on serum IgE levels was 52% in the systemic-reaction group and 0% in the oral-reaction group (P < 0.05). In the systemic reaction group, there was no significant difference in reactivity between nPru p 7 and prPru p 7, but the reactivity of erPru p 7 was significantly lower than those of nPru p 7 and prPru p 7 (P < 0.05).


Conclusions: We found that prPru p 7 exhibited reactivity in ELISA comparable to that of nPru p 7 for the diagnosis of peach allergy with systemic reaction.

Abstract 77 | PDF Downloads 127

References

1. Ortolani C, Ispano M, Pastorello E, Bigi A, Ansaloni R. The oral allergy syndrome. Ann Allergy. 1988;61 Pt 2:47-52.

2. Ortolani C, Ispano M, Pastorello EA, Ansaloni R, Magri GC. Comparison of results of skin prick tests (with fresh foods and commercial food extracts) and RAST in 100 patients with oral allergy syndrome. J Allergy Clin Immunol. 1989;83:683-90,
http://dx.doi.org/10.1016/0091-6749(89)90083-3.

3. Fernández-Rivas M, Van Ree R, Cuevas M. Allergy to Rosaceae fruits without related pollinosis. J Allergy Clin Immunol. 1997;100:728-33, http://dx.doi.org/10.1016/S0091-6749(97)70265-3.

4. Cuesta-Herranz J, Lázaro M, de las Heras M, Lluch M, Figueredo E, Umpierrez A, et al. Peach allergy pattern: experience in 70
patients. Allergy. 1998;53:78-82.

5. Pastorello EA, Incorvaia C, Pravettoni V, Farioli L, Conti A, Vigano G, et al. New allergens in fruits and vegetables. Allergy. 1998;53 Suppl.:48-51.

6. Inomata N, Okazaki F, Moriyama T, Nomura Y, Yamaguchi Y, Honjoh T, et al. Identification of peamaclein as a marker allergen related to systemic reactions in peach allergy. Ann Allergy Asthma Immunol. 2014;112, http://dx.doi.org/10.1016/j.anai.2013.11.003, 175-7.e3.

7. Fernández-Rivas M, González-Mancebo E, Rodríguez-Pérez R, Benito C, Sánchez-Monge R, Salcedo G, et al. Clinically relevant peach allergy is related to peach lipid transfer protein, Pru p 3, in the Spanish population. J Allergy Clin Immunol. 2003;112:789-95, http://dx.doi.org/10.1016/S0091.

8. Ebo DG, Bridts CH, Verweij MM, De Knop KJ, Hagendorens MM, De Clerck LS, et al. Sensitization profiles in birch pollen-allergic patients with and without oral allergy syndrome to apple: lessons from multiplexed component resolved allergy diagnosis. Clin Exp Allergy. 2010;40:339-47, http://dx.doi.org/10.1111/j.1365-2222.2009.03345.x.

9. Pastorello EA, Farioli L, Pravettoni V, Ortolani C, Ispano M, Monza M, et al. The major allergen of peach (Prunus persica) is a lipid transfer protein. J Allergy Clin Immunol. 1999;103:520-6, http://dx.doi.org/10.1016/S0091-6749(99)70480-X.

10. Rossi RE, Monasterolo G, Canonica GW, Passalacqua G. Systemic reactions to peach are associated with high levels of specific IgE to Pru p 3. Allergy. 2009;64:1795-6, http://dx.doi.org/10.1111/j.1398-9995.2009.02133.x.

11. Pascal M, Munoz-Cano ˜ R, Reina Z, Palacín A, Vilella R, Picado C, et al. Lipid transfer protein syndrome: clinical pattern, cofactor effect and profile of molecular sensitization to plant-foods and pollens. Clin Exp Allergy. 2012;42:1529-39, http://dx.doi.org/10.1111/j.1365-2222.2012.04071.x.

12. Pastorello EA, Farioli L, Pravettoni V, Scibilia J, Mascheri A, Borgonovo L, et al. Pru p 3-sensitised Italian peach-allergic patients are less likely to develop severe symptoms when also presenting IgE antibodies to Pru p 1 and Pru p 4. Int Arch Allergy Immunol. 2011;156:362-72, http://dx.doi.org/10.1159/000324440.

13. Amelio CMD, Goikoetxea MJ, Martínez-aranguren R, García BE, Gómez F, Fernández J, et al. Is the performance of Immuno CAP ISAC 112 sufficient to diagnose peach and apple allergies? Ann Allergy Asthma Immunol. 2016;116:2015-6, http://dx.doi.org/10.1016/j.anai.2015.11.003.

14. Fernández-Rivas M, Cuevas M. Peels of Rosaceae fruits have a higher allergenicity than pulps. Clin Exp Allergy. 1999;29:1239-47, http://dx.doi.org/10.1046/j.1365-2222.1999.00628.x.

15. Tuppo L, Alessandri C, Pomponi D, Picone D, Tamburrini M, Ferrara R, et al. Peamaclein - A new peach allergenic protein: similarities, differences and misleading features compared to Pru p 3. Clin Exp Allergy. 2013;43:128-40, http://dx.doi.org/10.1111/cea.12028.

16. Inomata N, Miyakawa M, Aihara M. Eyelid edema as a predictive factor for sensitization to Pru p 7 in peach allergy. J Dermatol. 2016, http://dx.doi.org/10.1111/1346-8138.13316.

17. Tuppo L, Spadaccini R, Alessandri C, Wienk H, Boelens R, Giangrieco I, et al. Structure, stability, and IgE binding of the peach allergen Peamaclein (Pru p 7). Biopolymers. 2014;102:416-25, http://dx.doi.org/10.1002/bip.22530.

18. Chapman MD, Smith AM, Vailes LD, Arruda LK, Dhanaraj V, Pomés A. Recombinant allergens for diagnosis and therapy of allergic disease. J Allergy Clin Immunol. 2000;106:409-18, http://dx.doi.org/10.1067/mai.2000.109832.

19. Novembre E, Mori F, Contestabile S, Rossi ME, Pucci N. Correlation of anti-pru p 3 IgE levels with severity of peach allergy reactions in children. Ann Allergy Asthma Immunol. 2012;108:271-4, http://dx.doi.org/10.1016/j.anai.2012.02.006.

20. Klein C, de Lamotte-Guéry F, Gautier F, Moulin G, Boze H, Joudrier P, et al. High-level secretion of a wheat lipid transfer protein in Pichia pastoris. Protein Expr Purif. 1998;13:73-82, http://dx.doi.org/10.1006/prep.1998.0888.

21. Hiller KM, Lubahn BC, Klapper DG. Cloning and expression of ragweed allergen Amb a 6. Scand J Immunol. 1998;48:26-36, http://dx.doi.org/10.1046/j.1365-3083.1998.00355.x.

22. Diaz-Perales A, Garcia-Casado G, Sanchez-Monge R, GarciaSelles FJ, Barber D, Salcedo G. cDNA cloning and heterologous expression of the major allergens from peach and apple belonging to the lipid-transfer protein family (vol 32, pg 87, 2002). Clin Exp Allergy. 2002;32:1387.

23. Ahrazem O, Ibánez ˜ MD, López-Torrejón G, Sánchez-Monge R, Sastre J, Lombardero M, et al. Lipid transfer proteins and allergy to oranges. Int Arch Allergy Immunol. 2005;137:201-10, http://dx.doi.org/10.1159/000086332.

24. Zuidmeer L, Salentijn E, Rivas MF, Mancebo EG, Asero R, Matos CI, et al. The role of profilin and lipid transfer protein in strawberry allergy in the Mediterranean area. Clin Exp Allergy. 2006;36:666-75, http://dx.doi.org/10.1111/j.1365-2222.2006.02453.x.

25. Lauer I, Alessandri S, Pokoj S, Reuter A, Conti A, Vieths S, et al. Expression and characterization of three important panallergens from hazelnut. Mol Nutr Food Res. 2008;52 Suppl. 2., http://dx.doi.org/10.1002/mnfr.200700426.

26. Pokoj S, Lauer I, Fötisch K, Himly M, Mari A, Enrique E, et al. Pichia pastoris is superior to E. coli for the production of recombinant allergenic non-specific lipid transfer proteins. Protein Expr Purif. 2010;69:68-75, http://dx.doi.org/10.1016/j.pep.2009.08.014.

27. Skypala IJ, Bull S, Deegan K, Gruffydd-Jones K, Holmes S, Small I, et al. The prevalence of PFS and prevalence and characteristics of reported food allergy; a survey of UK adults aged 18-75 incorporating a validated PFS diagnostic questionnaire. Clin Exp Allergy. 2013;43:928-40, http://dx.doi.org/10.1111/cea.12104.

28. Hirose J, Kitabatake N, Kimura A, Narita H. Recognition of native and/or thermally induced denatured forms of the major food allergen, ovomucoid, by human IgE and mouse monoclonal IgG antibodies. Biosci Biotechnol Biochem. 2004;68:2490-7, http://dx.doi.org/10.1271/bbb.68.2490.

29. Gamboa PM, Cáceres O, Antepara I, Sánchez-Monge R, Ahrazem O, Salcedo G, et al. Two different profiles of peach allergy in the north of Spain. Allergy. 2007;62:408-14, http://dx.doi.org/10.1111/j.1398-9995.2006.01284.x.

30. Díaz-Perales A, Sanz ML, García-Casado G, Sánchez-Monge R, García-Selles FJ, Lombardero M, et al. Recombinant Pru p 3 and natural Pru p 3, a major peach allergen, show equivalent immunologic reactivity: a new tool for the diagnosis of fruit allergy. J Allergy Clin Immunol. 2003;111:628-33, http://dx.doi.org/10.1067/mai.2003.75.

31. Asero R, Mistrello G, Roncarolo D, Amato S, Caldironi G, Barocci F, et al. Immunological cross-reactivity between lipid transfer proteins from botanically unrelated plant-derived foods: a clinical study. Allergy. 2002;57:900-6, http://dx.doi.org/10.1034/j.1398-9995.2002.t01-1-23541.x.

32. Almasia NI, Bazzini AA, Hopp HE, Vazquez-Rovere C. Overexpression of snakin-1 gene enhances resistance to Rhizoctonia solani and Erwinia carotovora in transgenic potato plants. Mol Plant Pathol. 2008;9:329-38, http://dx.doi.org/10.1111/j.1364-3703.2008.00469.x.