Correlation of OX40 ligand on B cells with serum total IgE and IL-4 levels by CD4+ T cells in allergic rhinitis
Main Article Content
Keywords
OX40 ligand, Immunoglobulin E, Interleukin-4, Allergic rhinitis
Abstract
Introduction and objectives: Allergic rhinitis (AR) is a classic Th2-mediated disease, with important contributions to the pathology of interleukins 4, 5, and 13. The co-stimulatory molecule of OX40 and its ligand interaction participate in the immune response by regulation of Th1/Th2 cells balance. Considering the paucity of information on the relation between OX40 ligand (OX40L) and AR, this study aimed to examine its expression on B lymphocytes.
Patients and methods: This case-control study consisted of 20 AR patients and 20 healthy subjects. The serum level of total immunoglobulin E (IgE) was measured using the electro-chemiluminescence (ECL) technology. The percentage of B-lymphocytes expressing OX40L was assessed by flow cytometry. The amounts of IL-4 in CD4+ T cells culture supernatant was also measured by the enzyme-linked immunosorbent assay (ELISA).
Results: OX40L expression on B lymphocytes of patients was significantly higher than the control group (44.32 ± 19.21% vs. 2.79 ± 2.48% respectively, p < 0.001). In AR patients, OX40L expression correlated positively with the levels of serum total IgE and IL-4 produced by CD4+ T lymphocytes (p < 0.01 - p < 0.05) respectively.
Conclusions: Collectively, the findings of this work suggest that there is a relationship between the OX40L expression level on B lymphocytes and allergic markers such as IgE and IL-4 in patients with allergic rhinitis.
References
2. Min Y-G. The pathophysiology, diagnosis and treatment of allergic rhinitis. Allergy Asthma Immunol Res. 2010;2:65-76.
3. Shaaban R, Zureik M, Soussan D, Neukirch C, Heinrich J, Sunyer J, et al. Rhinitis and onset of asthma: a longitudinal populationbased study. The Lancet. 2008;372:1049-57.
4. Quraishi SA, Davies MJ, Craig TJ. Inflammatory responses in allergic rhinitis: traditional approaches and novel treatment strategies. Am Osteopath Assoc. 2004;104 Suppl. 5:7-15.
5. Pawankar R, Mori S, Ozu C, Kimura S. Overview on the pathomechanisms of allergic rhinitis. Asia Pac Allergy. 2011;1:157-67.
6. Deo SS, Mistry KJ, Kakade AM, Niphadkar PV. Role played by Th2 type cytokines in IgE mediated allergy and asthma. Lung India. 2010;27:66.
7. Willoughby J, Griffiths J, Tews I, Cragg MS. OX40: structure and function - what questions remain? Mol Immunol. 2017;83:13-22.
8. Croft M. Control of immunity by the TNFR-related molecule OX40 (CD134). Annu Rev Immunol. 2009;28:57-78.
9. Kondo K, Okuma K, Tanaka R, Zhang LF, Kodama A, Takahashi Y, et al. Requirements for the functional expression of OX40 ligand on human activated CD4+ and CD8+ T cells. Hum Immunol. 2007;68:563-71.
10. Ishii N, Takahashi T, Soroosh P, Sugamura K. OX40-OX40 ligand interaction in T-cell-mediated immunity and immunopathology. Adv Immunol. 2010;105:63-98.
11. Voo KS, Bover L, Harline ML, Vien LT, Facchinetti V, Arima K, et al. Antibodies targeting human OX40 expand effector T cells and block inducible and natural regulatory T cell function. J Immunol. 2013;191:3641-50.
12. Eifan A, Durham S. Pathogenesis of rhinitis. Clin Exp Allergy. 2016;46:1139-51.
13. Hori T. Roles of OX40 in the pathogenesis and the control of diseases. Int J Hematol. 2006;83:17.
14. Chung D, Park K, Yarlagadda B, Davis EM, Platt M. The significance of serum total immunoglobulin E for in vitro diagnosis of allergic rhinitis. Int Forum Allergy Rhinol. 2014.
15. Farres MN, Sabry MK, Ahmed EE, Elkady HM, Mohamed NA. OX40 ligand: a potential costimulatory molecule in atopic asthma. J Asthma. 2014;51:573-7.
16. Burgess JK, Blake AE, Boustany S, Johnson PR, Armour CL, Black JL, et al. CD40 and OX40 ligand are increased on stimulated asthmatic airway smooth muscle. J Allergy Clin Immunol. 2005;115:302-8.
17. Burgess JK, Carlin S, Pack RA, Arndt GM, Au WW, Johnson PR, et al. Detection and characterization of OX40 ligand expression in human airway smooth muscle cells: a possible role in asthma? J Allergy Clin Immunol. 2004;113:683-9.
18. Hoshino A, Tanaka Y, Akiba H, Asakura Y, Mita Y, Sakurai T, et al. Critical role for OX40 ligand in the development of pathogenic Th2 cells in a murine model of asthma. Eur J Immunol. 2003;33:861-9.
19. Ezzat MHM, Imam SS, Shaheen KYA, Elbrhami EM. Serum OX40 ligand levels in asthmatic children: a potential biomarker of severity and persistence. Allergy Asthma Proc. 2011;32:313-8.
20. Lei W, Zhu CH, Zeng da XWQ, Zhang X, Chen Y, Mu C, et al. SOX40L: an important inflammatory mediator in adult bronchial asthma. Ann Acad Med Singapore. 2012;41:200-4.
21. Ezzat MH, Sallam MA, Shaheen KY, Abo-El-Haythim RE. Serum OX40 ligand: a potential marker of atopic dermatitis disease severity in children. Egypt J Pediatr Allergy Immunol. 2009;7.
22. Ilves T, Harvima I. OX40 ligand and OX40 are increased in atopic dermatitis lesions but do not correlate with clinical severity. J Eur Acad Dermatol Venereol. 2013;27.
23. Croft M, So T, Duan W, Soroosh P. The significance of OX40 and OX40L to T-cell biology and immune disease. Immunol Rev. 2009;229:173-91.
24. Hazra A, Gogtay N. Biostatistics series module 6: correlation and linear regression. Indian J dermatol. 2016;61:593.
25. Ohashi Y, Nakai Y, Okamoto H, Ohno Y, Sakamoto H, Sugiura Y, et al. Serum level of interleukin-4 in patients with perennial allergic rhinitis during allergen-specific immunotherapy. Scand J Immunol. 1996;43:680-6.
26. Siddiqui S, Mistry V, Doe C, Stinson S, Foster M, Brightling C. Airway wall expression of OX40/OX40L and interleukin-4 in asthma. Chest. 2010;137:797-804.
27. Linton P-J, Bautista B, Biederman E, Bradley ES, Harbertson J, Kondrack RM, et al. Costimulation via OX40L expressed by B cells is sufficient to determine the extent of primary CD4 cell expansion and Th2 cytokine secretion in vivo. J Exp Med. 2003;197:875-83.
28. Deraz TE. Immunopathogenesis of allergic rhinitis. Egypt J Pediatr Allergy Immunol. 2010;8.
29. Meng Q, Liu X, Li P, He L, Xie J, Gao X, et al. The influence of house dust mite sublingual immunotherapy on the TSLP-OX40L signaling pathway in patients with allergic rhinitis. Int Forum Allergy Rhinol. 2016;6:862-70.
30. Huang L, Ji W, Zhou W, Shi Q, Chen X, Hu Y. Effects of costimulatory pathway OX40/OX40L on the pathogenesis of allergic
asthma in mice. Zhonghua Er Ke Za Zhi. 2006;44:455-8.
Article Sidebar
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.