Cross-reactivity of Can f 1 with Syrian hamster and Fel d 1 in children

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Jeongmin Lee
Se-ah Jeon
Sooyoung Lee


Syrian hamster; dog allergy; cross-reactivity


Introduction and objectives: With increasing pet allergies among pediatric patients, the need for precise environmental care is increasing. We investigated the clinical, immunological, and environmental characteristics of pediatric patients sensitized to a dog to evaluate the cross-antigenicity of canine lipocalin Can f 1 with feline lipocalin Fel d 1 and Syrian hamster
Materials and methods: The protein fractions of the processed and commercial Syrian hamster extracts were compared using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). An enzyme-linked immunosorbent assay (ELISA) inhibition test was performed on Can f 1, Fel d 1, and processed Syrian hamster extract, and the antigen-specific immunoglobulin E (IgE)-binding capacity for each antigen was analyzed using serum samples from patients.
Results: Twelve of 19 patients with a median age of 40.5 months were symptomatic when exposed to dogs. Eleven (91.7%) patients showed a positive IgE response to Can f 1. Two patients were positive for Fel d 1-specific IgE antibody, and one was positive for hamster-specific IgE antibody. SDS-PAGE confirmed the presence of different patterns of protein bands
between the commercial and processed hamster extracts. There was no cross-antigenicity among Can f 1, Fel d 1, and processed Syrian hamster extract.
Conclusions: Since the standard commercial hamster extract did not contain Syrian hamster antigens that were diverse enough, caution should be taken when using it. In children allergic to cats and dogs, sensitization to isolated Can f 1 or Fel d 1 is unlikely to cause cross-reactivity to Syrian hamster hair and epithelium.

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1. Yang MS, Lee SP, Kwon YJ, Lee SM. Dog and cat allergies and allergen avoidance measures in Korean adult pet owners who participated in a pet exhibition. Allergy Asthma Immunol Res. 2018;10:155–64.
2. Gabet S, Just J, Couderc R, Seta N, Momas I. Allergic sensitisation in early childhood: Patterns and related factors in
PARIS birth cohort. Int J Hyg Environ Health. 2016;219:792– 800.
3. Kim J, Hahm MI, Lee SY, Kim WK, Chae Y, Park YM, et al. Sensitization to aeroallergens in Korean children: A population-
based study in 2010. J Korean Med Sci. 2011;26:1165–72.
4. Konradsen JR, Fujisawa T, van Hage M, Hedlin G, Hilger C, Kleine-Tebbe J, et al. Allergy to furry animals: New insights,
diagnostic approaches, and challenges. J Allergy Clin Immunol. 2015;135:616–25.
5. Nilsson OB, van Hage M, Gronlund H. Mammalian-derived respiratory allergens – implications for diagnosis and therapy
of individuals allergic to furry animals. Methods. 2014;66:86–95.
6. Curin M, Reininger R, Swoboda I, Focke M, Valenta R, Spitzauer S. Skin prick test extracts for dog allergy diagnosis
show considerable variations regarding the content of major and minor dog allergens. Int Arch Allergy Immunol.
7. Chan SK, Leung DYM. Dog and cat allergies: Current state of diagnostic approaches and challenges. Allergy Asthma Immunol Res. 2018;10:97–105.
8. Anna A, Carl H, Konrad W, Christian L, Niklas A, Inger K, et al. Sensitization to cat and dog allergen molecules in childhood
and prediction of symptoms of cat and dog allergy in adolescence:ABAMSE/MeDALL study. J Allergy Clin Immunol.
9. Berto JM, Pelaez A, Fernandez E, Lombardero M, Ferrer M. Siberian hamster: A new indoor source of allergic sensitization
and respiratory disease. Allergy. 2002;57:155–9.
10. Phillips JF, Lockey RF. Exotic pet allergy. J Allergy Clin Immunol. 2009;123:513–15.
11. Lim DL, Chan RM, Wen H, Van Bever HP, Chua KY. Anaphylaxis after hamster bites – Identification of a novel
allergen. Clin Exp Allergy. 2004;34:1122–3.
12. Torres JA, de Las Heras M, Maroto AS, Vivanco F, Sastre J, Pastor-Vargas C. Molecular and immunological characterization
of the first allergenic lipocalin in hamster: The major allergen from Siberian hamster (Phodopus sungorus). J Biol Chem. 2014;289:23382–8.
13. Torres JA, Pastor-Vargas C, de las Heras M, Vivanco F, Cuesta J, Sastre J. An odorant-binding protein as a new allergen from Siberian hamster (Phodopus sungorus). Int Arch Allergy Immunol. 2012;157:109–12.
14. Hilger C, Dubey VP, Lentz D, Davril C, Revets D, Muller CP, et al. Male-specific submaxillary gland protein, a lipocalin allergen of the golden hamster, differs from the lipocalin allergens of Siberian and Roborovski dwarf hamsters. Int Arch Allergy Immunol. 2015;166:30–40.
15. Luo S, Sun Y, Hou J, Kong X, Wang P, Zhang Q, et al. Pet keeping in childhood and asthma and allergy among children in
Tianjin area, China. PLoS One. 2018;13:e0197274.
16. Torres JA, Sastre J, Vivanco F, Sanz Maroto A, de Las Heras M, Pastor-Vargas C. Clinical characteristics of patients sensitized to Siberian hamster. J Investig Allergol Clin Immunol. 2016;26:113–115.
17. Apostolovic D, Sanchez-Vidaurre S, Waden K, Curin M, Grundstrom J, Gafvelin G, et al. The cat lipocalin Fel d 7 and
its cross-reactivity with the dog lipocalin Can f 1. Allergy. 2016;71:1490–5.
18. Liccardi G, Salzillo A, Calzetta L, Piccolo A, Puxeddu E, Rogliani P. Is allergic sensitization to Siberian hamster preventable
in high-risk individuals who are already sensitized or exposed to furry animals? J Investig Allergol Clin Immunol. 2016;26:403–5.